Objective: To study the chemical constituents and antibacterial activity of essential oil from Adenosma indianum Method: The essential oils were extracted from Adenosma indianum by steam distillation. The amount of the constituents from the essential oil was determined by normalization method

and the constituents separated were identified by GC-MS. The antibacterial tests of MIC and MBC for the essential oil were studied by using tubes serial dilution method. Result: There were 57 constituents from Adenosma indianum by SD that composed of about 92.19% of the total essential oil were separated and identified. It mainly contained cedrol (26.02%) and limonene (13.46%). The essential oil showed some antibacterial effect to staphylococcus aureus and drug-resistant strains of staphylococcus aureus

etc. Conclusion: It can provide scientific basis for the effective substances of A.indianum.