Objective: To analyze the difference of the saponins in the different processed products of Radix Polygalae(crude Radix Polygalae

licorice And Honey Processed Radix Polygalae). Method: The content of polygalacic acid and senegenin in the different processed products of Radix Polygalae was determine by HPLC using an Agilent ZORBAX SB-C18(4.6 mm×250 mm

5 μm) with acetonitrile-0.1% phosphoric acid (41 ∶59) as the mobile phase; the flow rate was 1.0 mL ·min-1; temperatures was 25 ℃ and UV detection wavelength at 210 nm. Result: The order of the content of polygalacic acid and senegenin (from higher to lower) was:crude Radix Polygalae

licorice processed Radix Polygalae

honey processed Radix Polygalae. Conclusion: The different process method has some effects on the content of saponins in Radix Polygalae. The content of polygalacic acid and senegenin in honey processed Radix Polygalae was the lowest. The LD50 experiment result suggests that honey processed can reduce the toxic composition of crude Radix Polygalae .