响应面法优化黄花菜总黄酮提取工艺

周向军; 高义霞; 张霞

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响应面法优化黄花菜总黄酮提取工艺

更新时间：2024-01-04

- 响应面法优化黄花菜总黄酮提取工艺
- Optimization of Extraction Technology of Total Flavonoids from by Response Surface Method
- 中国实验方剂学杂志 2011年17卷第16期页码：29-32
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- 纸质出版日期：2011
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周向军, 高义霞, 张霞. 响应面法优化黄花菜总黄酮提取工艺[J]. 中国实验方剂学杂志, 2011,17(16):29-32.

ZHOU Xiang-jun, GAO Yi-xia, ZHANG Xia. Optimization of Extraction Technology of Total Flavonoids from by Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 29-32.
周向军, 高义霞, 张霞. 响应面法优化黄花菜总黄酮提取工艺[J]. 中国实验方剂学杂志, 2011,17(16):29-32. DOI：

ZHOU Xiang-jun, GAO Yi-xia, ZHANG Xia. Optimization of Extraction Technology of Total Flavonoids from by Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 29-32. DOI：

摘要

目的: 采用响应面法优化黄花菜总黄酮的提取工艺。方法: 在单因素试验的基础之上

选取浸提温度、浸提时间、料液比和乙醇体积分数作为影响因子

应用Box-Behnken中心组合设计建立数学模型

以总黄酮提取率为响应值

进行响应面分析。结果: 黄花菜总黄酮最佳提取工艺为90%乙醇浸提时间180 min

浸提温度75 ℃

料液比1∶35。总黄酮提取率预测值为0.571%

实际值为0.581%

相对误差为1.32%。结论: 优选工艺工艺稳定、可行

操作简单。

Abstract

Objective: Response surface analysis methodology(RSM) was used for optimizing the extraction technology of total flavonoids from Hemerocallis citrina. Method: On the basis of single-factor tests

extraction temperature

extraction time

solid-liquid ratio and ethanol concentration were selected as influencing factors during extraction. Box-Behnken design was used to establish mathematical model. Result: The optimum conditions for total flavonoids were extraction time 180 min

extraction temperature 75 ℃

ethanol concentration 90%

solid-liquid ratio 1∶35. The predicted extraction yield of total flavonoids was 0.571%

and the verification 0.581%

with relative error of 1.32%. Conclusion: The optimized extraction technology was stable

feasible and simple.

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