ZOU Bing, LIU Yu-qiang, CAI Qian. Determination of Catechin in Crude Drugs from Various Habitats and Prepared Medical Herbs of Rupr. from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 68-70.
ZOU Bing, LIU Yu-qiang, CAI Qian. Determination of Catechin in Crude Drugs from Various Habitats and Prepared Medical Herbs of Rupr. from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 68-70.DOI:
Objective: To determine the content of catechin in crude drugs from various habitats and prepared medical herbs of Cynomorium songaricum from different areas by HPLC. Method: The determination was carried on Tigerkin C18column
0.04 mol ·L-1 citric acid-N
N-dimethylformamide-tetrahydrofuran (52 ∶5 ∶1) as mobile phase with a flow rate 1.0 mL ·min-1 and detection wavelength at 280 nm. Result: Among the crude drugs from 10 various habitats
the content of catechin in crude drugs from 9 various habitats is over 0.080% except from gannan which was 0.048%; Among the prepared medical herbs from 22 regions
the content of catechin in 5 various habitats is over 0.080%
the content in most prepared medical herbs was over 0.040%. Conclusion: The contents of catechin in crude drugs from various habitats and prepared medical herbs of Herbe cynomorii from different areas were different
the content of catechin in prepared medical herbs was lower than that in crude drugs.