不同溶剂对鸡血藤提取物总黄酮含量 及抗肿瘤活性的影响

程悦; 王志宇; 王冬梅; 刘浩文; 陈建萍

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不同溶剂对鸡血藤提取物总黄酮含量及抗肿瘤活性的影响

更新时间：2024-01-04

- 不同溶剂对鸡血藤提取物总黄酮含量及抗肿瘤活性的影响
- Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from
- 中国实验方剂学杂志 2011年17卷第9期页码：142-145
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- 纸质出版日期：2011
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程悦, 王志宇, 王冬梅, 等. 不同溶剂对鸡血藤提取物总黄酮含量及抗肿瘤活性的影响[J]. 中国实验方剂学杂志, 2011,17(9):142-145.

CHENG Yue, WANG Zhi-yu, WANG Dong-mei, et al. Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 142-145.
程悦, 王志宇, 王冬梅, 等. 不同溶剂对鸡血藤提取物总黄酮含量及抗肿瘤活性的影响[J]. 中国实验方剂学杂志, 2011,17(9):142-145. DOI：

CHENG Yue, WANG Zhi-yu, WANG Dong-mei, et al. Total Flavonoids and its Anti-tumor Activities by Different Solvent Extract from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 142-145. DOI：

摘要

目的: 探讨不同溶剂对鸡血藤提取物中的总黄酮含量和抗肿瘤活性的影响。方法: 分别用不同浓度的乙醇水溶液、丙酮水溶液作为提取溶剂

利用回流法、超声提取法制备鸡血藤提取物;采用四甲基噻唑蓝(MTT)法测定提取物对结肠癌HT-29细胞增殖的抑制活性;采用分光光度法测定提取物中总黄酮含量;采用HPLC法测定提取物中芒柄花素含量。结果: 60%乙醇回流60 min所得提取物总黄酮含量最高并且对HT-29细胞增殖的抑制活性最强。结论: 鸡血藤抗肿瘤活性提取物的制备以60%乙醇回流提取为佳。

Abstract

Objective: To study the total flavonoids and anti-tumor activities of different solvent extract from Spatholobus suberectus and determine the extraction solvent and extraction method. Method: Using ethanol or acetone aqueous solution as the solvent

different extracts were prepared from S. suberectus by refluxing or ultrasonic extraction.The inhibitory activity on the proliferation of HT-29 cancer cell was determined by MTT;The content of total flavonoids and formononetin were determined by spectrophotometry and HPLC

respectively. Result : The content of total flavonoids and the cytotoxic activity were highest in the 60% ethanol 60 min-refluxing extract. Conclusion : Using 60% ethanol as extraction solvent

refluxing as extraction method

obtained extract from S. suberectus showed highest anti-tumor activity on HT-29 cancer cell.

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