LIU Wei, GUO Lei, CUI Yong-xia, et al. Content Determination of Total Scopoletin in Wild and Cultivated from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 73-75.
LIU Wei, GUO Lei, CUI Yong-xia, et al. Content Determination of Total Scopoletin in Wild and Cultivated from Different Areas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 73-75.DOI:
Objective: To establish an HPLC method for content determination of total scopoletin in wild and cultivated Cortex Lycii from different areas. Method: The separation was performed on a column C18
the mobile phase was acetonitrile-1% acetic acid (15 ∶85);the flow rate was 0.8 mL ·min-1;the column temperature was at 30 ℃; the detective wavelength was at 345 nm. Result: The linear range of scopoletin was from 6 to 48 mg ·L-1.The average recovery was 97.19% (RSD 1.77%). Conclusion: The method is reliable