ZHANG Yi-ming, WANG Jie, HU Ming-xun, et al. Determination of Four Active Components in Hongyao Strapping by Capillary Gas Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 47-50.
ZHANG Yi-ming, WANG Jie, HU Ming-xun, et al. Determination of Four Active Components in Hongyao Strapping by Capillary Gas Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 47-50.DOI:
目的: 建立毛细管气相色谱法同时测定红药贴膏中樟脑、薄荷脑、冰片和水杨酸甲酯的含量。 方法: 采用毛细管气相色谱法。色谱柱为PEG-20M石英毛细管柱(0.25 mm×0.25 μm×30 m)
氢火焰离子检测器
检测器和气化室的温度均为200 ℃
柱温160 ℃
氮气为载气
正辛醇为内标物
用挥发油测定器蒸馏制备供试品溶液。 结果: 在该色谱条件下
樟脑、薄荷脑、冰片和水杨酸甲酯分离度和线性关系良好
回收率分别为99.8%
100.3%
99.5%
99.7%。 结论: 该方法简便、准确、专属性强、重复性好
可用于红药贴膏的质量控制。
Abstract
Objective: To establish a method for determination of camphor
menthol
borneol and methylsalicylate in Hongyao strapping. Method: Capillary gas chromatography was adopted. The chromatography separation was carried out with PEG-20M capillary column(0.25 μm×0.25 mm×30 m)
using a flame ionization detector. The injector and detector temperature were set at 200 ℃. The column temperature was 160 ℃. n-Octanol was used as internal standard. The sample solution was prepared by distillation with essential oil collector. Result: The resolution and linearity of camphor
menthol
borneol and methylsalicylate were good under the chromatographic conditions. The average recoveries of camphor
menthol
borneol and methylsalicylate were 99.8%
100.3%
99.5%
99.7%. Conclusion: This method is simple
sensitive
accurate and has good repeatability for controlling the quality of camphor
menthol
borneol and methylsalicylate in Hongyao strapping.