Objective: To achieve rapid quantitation of active components in Tongmai granule. Method: By choosing Kinetex C18 (3 mm×50 mm

2.6 μm) as analysis column

an HPLC-UV method was established to detect puerarin and ferulic acid in Tongmai Granule under detection wavelength of 313 nm. The mobile phrase was optimized as 10% acetonitrile-1% acetic acid (54 ∶46) at flow rate of 0.6 mL ·min-1. Result: Under this analytic condition

the quantitation could be accomplished within 10 minutes profiling good separation and specificity of puerarin and ferulic acid which presented retention time at 5.7

7.6 min.The average recovery was (98.1±1.40) %

(103.7±1.68) % for puerarin and ferulic acid respectively. Calibration curves ranged from 10 to 100 mg ·L-1 for puerarin and from 0.05 to 0.5 mg ·L-1 for ferulic acid with correlation coefficients of 0.999 7 and 0.999 3. Conclusion: The method is rapid

sensitive

accurate and precise. It could be used to provide a rapid determination in quality control of Tongmai granule.