Objective: To establish an HPLC method for simultaneous determination of ephedrine hydrochloride and morphine in Keke capsule. Method: The analysis carried out on a Sino Chrom ODS-BP C18(4.6 mm×250 mm

5 μm).Mobile phase comprised with methyl cyanides

0.01 mol ·L-1 potassium dihydrogen phosphate

phosphoric acid and triethanolamine under a ratio as 5 ∶95 ∶0.2 ∶0.4.Detect wave-length was at 222 nm.Flow rate was 1.0 mL ·min-1under room column temperature.Sample size was 10 μL. Result: The calibration curves were linear over the range of 0.16-0.8 g ·L-1 for ephedrine hydrochloride and 0.012-0.06 g ·L-1 for morphine respectively.The average recoveries were 99.09% for ephedrine hydrochloride with RSD 1.24% and 98.44% for morphine with RSD 1.45% respectively. Conclusion: The determination method is rapid

simple

and repeatable

and can be used for the quality control of Keke capsule.