Objective: To study the cleaning mechanism of Aconitum carmichaeli. Method: Titration method was used to determine the content of total alkaloids; UV was used to determine the content of ester-type alkaloids; HPLC was used to determine the content of three kind of diester alkaloids namely aconitine

hypaconitine

mesaconition; sulfuric-phenol method was used to determine the content of polysaccharides. Result: The content of total alkaloids in parent root

daughter root

rootlet of Aconitum carmichaeli was 11.20

11.70

18.40 mg ·g-1 respectively; the content of ester-type alkaloids was 4.41

3.33

8.24 mg ·g-1 respectively; the content of three kind of diester alkaloids was 0.90

1.07

2.18 mg ·g-1 respectively; the content of polysaccharides was 60.60

98.20

61.80 mg ·g-1 respectively. Conclusion: The categories of main chemical compositions of parent root

daughter root and rootlet have no obvious difference

but the content of them is different. The sum of content of three kind of diester alkaloids of rootlet is more than twice that of parent root.Cleaning has scientific value.