LIU Chang-qing, HE Bai-yin, FENG Feng, et al. Study on Extract Process of TanshinoneⅡ in[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 32-34.
LIU Chang-qing, HE Bai-yin, FENG Feng, et al. Study on Extract Process of TanshinoneⅡ in[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 32-34.DOI:
考察了提取时间、提取次数、乙醇倍量及乙醇体积分数对丹参酮ⅡA提取率的影响。 结果: 丹参药渣中丹参酮ⅡA的最佳提取工艺条件为:8倍量90%乙醇浸泡30 min
回流提取2次(85 ℃)
每次0.5 h
高效液相色谱法检测
水提后丹参药渣中丹参酮ⅡA的提取率约为91%。 结论: 优化的提取条件效率高
水提后丹参药渣中丹参酮ⅡA的含量较高
提示其可以作为一种丹参酮资源
具有开发利用的价值。
Abstract
Objective: To explore the best condition to extract tanshinone ⅡA in Salvia mihiorrhiza by ethano1. Method: The optimum condition was determined with L9 (34) orthogonal experiment to arrange experiments with extract rate of tanshinone ⅡA as quantitative index. Result: The best extraction process condition was: 8 times of 90 percent alcohol immersed for 30 minutes
reflux extraction was two times with each time of 0.5 hour at 85 ℃. The HPLC result showed that the extraction rate of tanshinone ⅡA was about 91%. Conclusion: Optimized extraction process design is reasonable
reproducible and can increase the reuse of herbs. Salvia mihiorrhiza can be served as a kind of tanshinone resource
which has great value of development and utilization.