Objective: To discuss the relationship between the liver fatty acid binding protein and kidney damage induced by aristolochic acid in rats evaluated by perfusion of isolated kidney. Method: Rat perfusion model of isolated kidney was established at the constant flow of 5 mL ·min-1.The control group was administrated with normal perfusate

while the experimental group was given aristolochic acid(7 mg in 50 mL).After 10 min of perfusion

the perfusate and urine at different time points were collected.Then concentration of K

Na

Cl was determined and their clearance was calculated.The content of liver fatty acid binding protein was detected by Elisa. Result: Compared with the control group

the clearance of K and Na in the experimental group given aristolochic acid began to decrease from (99.54±14.55)% and (27.93±6.66)% of pre-perfusion to (33.16±9.59)% and (9.70±2.62)% of 30 min after perfusion respectively

and liver fatty acid binding protein immediately increased significantly from (7.42±0.12)ng ·L-1 of pre-perfusion to (8.18±0.47)ng ·L-1 at the moment of after perfusion respectively. Conclusion: Liver fatty acid binding protein is superior to the clearance of traditional indicators in the evaluation of acute tubular injury induced by aristolochic acid.It is an early and sensitive biomarker of renal tubular injury.