Objective: To study the extraction technological parameters of the total alkaloids from Lycopodium japonicum. Method: The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated. Result: The optimized conditions in the process of acid water extraction were 0.5% hydrochloric acid as much as 15 times of the medicine amount for 24 h and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin 001×7 at 3 BV ·h-1 of flow rate

then eluted with 10 BV of 8% aqueous ammonia ethanol. Conclusion: This technology is rapid and accurate for the purification of total alkaloids from L.japonicum.