Objective: To establish a method of fingerprint analysis on Chrysanthemi Flos formula granules by HPLC. Method: The chromatographic fingerprints were obtained by HPLC with Waters Xbridge C18 column (4.6 mm×250 mm

5 μm) by gradient elution;the solvent system composed of acetonitrile-0.1% phosphric.The wavelength was set at 351 nm;the flow rate was 1.0 mL ·min-1 and the column temperature was maintained at 30 ℃. Result: Seventeen shared peaks were selected as the fingerprint peaks of Chrysanthemi Flos formula granules and good similarities with correlation coefficients higher than 0.85 were found in fingerprints between the different samples of Chrysanthemi Flos Formula Granules and the standard fingerprint. Conclusion :The method with good reproducibility is simple and accurate;it can be used as a quality control method for Chrysanthemi Flos formula granules.