HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷

何建雄; 赖小平; 魏刚; 左竣岭; 李辉标

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HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷

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- HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷
- Content Determination of Chlorogenic Acid, Paeoniflorin and Baicalin in Yinqiao Chaigui Decoction
- 中国实验方剂学杂志 2011年17卷第6期页码：48-50
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部"十一五"科技支撑计划资助项目(2006BA101B00)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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何建雄, 赖小平, 魏刚, 等. HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷[J]. 中国实验方剂学杂志, 2011,17(6):48-50.

HE Jian-xiong, LAI Xiao-ping, WEI Gang, et al. Content Determination of Chlorogenic Acid, Paeoniflorin and Baicalin in Yinqiao Chaigui Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 48-50.
何建雄, 赖小平, 魏刚, 等. HPLC测定银翘柴桂汤中绿原酸、芍药苷、黄芩苷[J]. 中国实验方剂学杂志, 2011,17(6):48-50. DOI：

HE Jian-xiong, LAI Xiao-ping, WEI Gang, et al. Content Determination of Chlorogenic Acid, Paeoniflorin and Baicalin in Yinqiao Chaigui Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 48-50. DOI：

摘要

目的: 建立银翘柴桂汤剂中黄芩苷、绿原酸、芍药苷的含量测定方法。方法: 采用HPLC法

色谱柱为Kromasil Cl8 (4.6mm×250 mm

5μm);流动相为甲醇-1%磷酸水(梯度洗脱);流速1 mL ·min-1;柱温为35 ℃;多波长检测芍药苷检测波长230nm

黄芩苷检测波长280 nm

绿原酸检测波长327 nm。结果: 绿原酸、芍药苷和黄芩苷在40 min内均能与其他组分实现良好分离

绿原酸、芍药苷和黄芩苷分别在0.077 6~0.776 0 μg(r=0.999 9)

0.076 1~0.761 0 μg(r=0.999 9)

0.264 8~2.64 8 μg(r=0.999 9)线性关系良好

加样回收率分别为102.67%(RSD 1.48

n=6)

97.85%(RSD 1.85

n=6)

101.86%(RSD 1.44

n=6)。结论: 方法简便、结果准确

能同时测定银翘柴桂汤中绿原酸、芍药苷和黄芩苷3种有效成分的含量。

Abstract

Objective: To develop an HPLC method for the Content determination of chlorogenic acid

paeoniflorin and baicalin inYinqiaochaigui decoction. Method :HPLC-based separation of the agents was performed on Kromasil Cl8 column (4.6 mm×250 mm

5 μm) at 25 ℃ with the mobile phase of methanol-0.1% Phosphoric acid water solution (gradient elution)

flow rate of 1.0 mL ·min-1 and multiple detection wavelength of 327

230

280 nm. Result :HPLC allowed simultaneous quantitative determination of the 3 components in Yinqiao Chaigui decoction

and they showed good linear relationships when their sample amount ranged 0.077 6-0.776 0 μg

0.076 1-0.761 0 μg and 0.264 8-2.648 μg

respectively

with correlation coefficients all beyond 0.999 9 and average recovery rates of 102.67%

97.85% and 101.86%

respectively. Conclusion :This method is simple and convenient in detecting the 3 components in Yinqiao Chaigui decoction

and the results are precise and reproducible.

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