Objective: To study the distribution of isorhynchophyline in liver and kidney tissues of SHR rats before and after compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Unics Le

to investigate the impact of channel tropism before and after combination. Method: Uncariae Ramulus Cum Uncis decoction group was administrated Uncariae Ramulus Cum Unics

Tianma Gouteng decoction group was administrated with decoction of compatible Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis at a dose of 1.172 g ·kg-1. A blank group was received normal saline. Then using HPLC to determinate the isorhynchophyline in liver and kidney tissues of SHR rats before and after the compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis. Analytical column was WatersTM(4.6 mm×250 mm

RP 18.5 μm). The mobile phase consisted of methanol-water (70 ∶30)

pH 8.0

rate was 10.8 mL · min-1. The detector wave length was set at 254 nm

the column temperature was 30 ℃. Result: Isorhynchophyline in liver and kidney tissues was linear in the range of 0.08-16.0 mg ·L-1. There existed significant (P<0.05) differences in liver and kidney tissues before and after the compatibility. Conclution :The compatibility of Chinese medicine has close correlation with channel tropism.