HPLC测定清胰利胆颗粒中绿原酸

夏莲; 陈卫卫

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HPLC测定清胰利胆颗粒中绿原酸

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- HPLC测定清胰利胆颗粒中绿原酸
- Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC
- 中国实验方剂学杂志 2011年17卷第10期页码：101-103
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- 纸质出版日期：2011
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夏莲, 陈卫卫. HPLC测定清胰利胆颗粒中绿原酸[J]. 中国实验方剂学杂志, 2011,17(10):101-103.

XIA Lian, CHEN Wei-wei. Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 101-103.
夏莲, 陈卫卫. HPLC测定清胰利胆颗粒中绿原酸[J]. 中国实验方剂学杂志, 2011,17(10):101-103. DOI：

XIA Lian, CHEN Wei-wei. Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 101-103. DOI：

摘要

目的: 建立清胰利胆颗粒中绿原酸的含量测定方法。方法: 采用高效液相色谱法

色谱柱welchrom-C18柱(4.6 mm×250 mm

5 μm);乙腈-0.1%磷酸(9 ∶91)为流动相

检测波长327 nm

流速1.0 mL ·min-1

柱温35 ℃。结果: 绿原酸含量在0.303 5~1.517 5 μg线性关系良好(r=0.999 7)

平均回收率为97.3%(RSD 1.9%)。结论: 本法操作简便、可靠

可用于清胰利胆颗粒的质量控制。

Abstract

Objective: To establish a method for determination of the content of chlorogenic acid in Qinyi Lidan granules by HPLC. Method :A HPLC method was used with welchrom C18 column(4.6 mm×250 mm

5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid(9 ∶91). The UV detector wavelength was at 327 nm.The flow rate was 1 mL ·min-1.The temperature of the column was at 35 ℃. Result: The calibration curve of chlorogenic acid was linear within the range of 0.303 5-1.517 5 μg(r=0.999 7). The average recovery was 97.3%(RSD 1.9%). Conclusion: The method is simple

convenient and reliable. It is appropriate for the content determination of the preparation.

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