Objective: To establish a quantification method for determination of quercetin

rutoside and kaempferol in Lespedeza virgata by HPLC. Method: RP-HPLC was applied on the determination of quercetin

rutoside and kaempferol in L.virgata with C18 column (4.6 mm×250 mm

5 μm)

DAD detector

and a mixture of acetonitrile-0.2% phosphoric acid (60 ∶40) as a mobile phase with the flow rate at 1.0 mL ·min-1.Three flavonoids were detected at 251 nm

column temperature was set at 35 ℃. Result: Quercetin

rutoside and kaempferol were linear in the range of 0.210-4.19

0.084-1.68

0.066-1.33 μg.The average recovery of rutoside

quercetin and kaempferol was 99.48%

98.53% and 99.33% with RSD (n=5) 0.51%

1.35% and 2.13% respectively. Conclution: The method is accurate and sensitive

which can be used rapidly for the content determinatinon of rutoside

quercetin and kaempferol from L.virgata.It can be applied to the quality control of this crude drug.