Objective: HPLC method prescribed in the main component of puerarin content. Method: HPLC to Kromasil C18(4.6 mm×250 mm

5 μm) column for the analysis of the mobile phase was methanol-water(27 ∶73)

flow rate 1 mL ·min-1

detection wavelength 250 nm

Result: puerarin regression equation Y=93 128X+92 301(r=0.999 5

n=6)

the scope of puerarin 0.06-0.18 μg a good linear relationship. The average recovery was 100.53%

RSD 1.41% precision. Conclusion: The method is simple

rapid

accurate

sensitive and suitable for quality control of Kanger skin.