Objective: Changes of chlorophyll

soluble sugar

soluble protein

during the plant regeneration in Rabdosia rubescens werestudied to explore the correlation between the accumulation of the active components and physiological characteristics of cultural materials of R. rubescens. Method: UV spectrophotometry and high performance liquid chromatography were used to determine the content of chlorophyll

soluble sugar

soluble protein in the cultural materials of different stage. Result: The content of chlorophyll

soluble protein and acetic acid was positively correlated with the oridonin

while the soluble sugar had a negative correlation with the oridonin. Conclusion: The content of chlorophyll

soluble protein in the cultural materials had a mutual promotion relationship with the oridonin

and the levels of soluble sugar were closely related to the growth speed and developmental phase of the cultural materials.