Objective: To study antimicrobial acitvity of Aquilaria sinensis peel extract. Method: The chloroform extract of A. sinensis peel was investigated for antimicrobial activity against the tested bacteria and fungi using filterpaper disc method and growth rate method respectively and their minimum inhibitory concentrations (MICs) were determined using serial dilution method. Result: The extract displayed significant antibacterial and antifungal activities against Staphylococcus aureus

Bacillus subtilis and Pseudomonas aeruginosa with MIC values of 6.25

6.25

12.5 g ·L-1 and against Trichoderma roseum

Aspergillus niger and A.flavus with MIC values of 6.25

6.25

12.5 g ·L-1. Conclusion: The chloroform extract of A. sinensis peel exhibited antimicrobial activity which is worthy of exploitation.