Objective: To investigate the chemical constituents from the petroleum ether and chloroform fractions of ethanol extract from Spiraea pubescens . Method: The compounds were isolated by silica gel chromatography and preparative HPLC

and their structures were elucidated on the basis of chemical and spectroscopic methods

including MS

1D and 2D NMR spectral techniques. Result: Six compounds were isolated from the petroleum ether and chloroform fractions of ethanol extract from S . pubescens

and were identified as lupeone(1)

friedelin(2)

lupeol(3)

β -amyrin(4)

betulin(5)

and uvaol(6). Conclusion: This is the first report of the isolation of 1-5 from Spiraea genus

and 6 from this plant.