TIAN Ming-yue, YU Li-chao, XU Xiu-quan. Optimization of Ultrasound Extraction of Total Triterpenoids from by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 31-33.
TIAN Ming-yue, YU Li-chao, XU Xiu-quan. Optimization of Ultrasound Extraction of Total Triterpenoids from by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 31-33.DOI:
Objective: To optimize the extraction technology of total triterpenoids from Inonotus obliquus by orthogonal test based on single-factor experiments. Method: The factors such as extraction solvent
extraction temperature
extraction time and material to solvent ratio were optimized by orthogonal test. Result: The factor of extraction time was the most significant parameter for the process
and then extraction temperature
sample to solvent ratio was the last one. The highest extracion rate of total triterpenoids by ultrasound-assisted extraction could arrive at 5.06% using isopropanol as solvent and the sample to solvent ratio was 1 ∶10 at 50 ℃ extracted for 30 min. Conclusion: The optimum extraction process for total triterpenoids was stable and feasible.