Objective: To estabish the HPLC method for determining the content of three flavonoids(calycosin

formononetin and rhamnocitrin)in the seeds of Astragali complanati. Method :An HPLC method has been developed to separate the three flavonoids on Kromasil C18(4.6 mm×250 mm

5 μm)column with methanol-1% acetic acid(56 ∶44)as mobile phase(1 mL ·min-1)

column temperature at 30 ℃ and UV detection wavelength at 266 nm. Result: The good linearity range of calycosin

formononetin and rhamnocitrin is 0.348-1.74(r=0.999 7)

0.288-1.44(r=0.999 8)

0.120-0.60(r=0.999 7). The average recovery of Calycosin

Formononetin and Rhamnocitrin are 100.3%(RSD 0.33%)

99.8%(RSD 2.78%)

98.6%(RSD 2.91%). Conclusion: The estabished method is simple

accurate and can be used for simultaneously determining the content of three flavonoids and for the the quality control of the seeds of A.complanati.