温下方含药血清诱导A549/DDP细胞凋亡及对Bcl-2,Bax,p53蛋白表达的影响

季旭明; 欧阳兵; 吴智春; 于华芸; 王春燕; 李文华

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温下方含药血清诱导A549/DDP细胞凋亡及对Bcl-2,Bax,p53蛋白表达的影响

更新时间：2024-01-04

- 温下方含药血清诱导A549/DDP细胞凋亡及对Bcl-2,Bax,p53蛋白表达的影响
- Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53
- 中国实验方剂学杂志 2011年17卷第12期页码：123-126
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30873222);山东省中青年科学家基金项目(2008BS03010);山东省中医药管理局科技发展计划项目(2009-016)
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- 纸质出版日期：2011
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季旭明, 欧阳兵, 吴智春, 等. 温下方含药血清诱导A549/DDP细胞凋亡及对Bcl-2,Bax,p53蛋白表达的影响[J]. 中国实验方剂学杂志, 2011,17(12):123-126.

JI Xu-ming, OU Yang-bing, WU Zhi-chun, et al. Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 123-126.
季旭明, 欧阳兵, 吴智春, 等. 温下方含药血清诱导A549/DDP细胞凋亡及对Bcl-2,Bax,p53蛋白表达的影响[J]. 中国实验方剂学杂志, 2011,17(12):123-126. DOI：

JI Xu-ming, OU Yang-bing, WU Zhi-chun, et al. Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 123-126. DOI：

摘要

目的: 研究温下方含药血清诱导人肺腺癌耐药细胞株A549/DDP凋亡及对Bcl-2

Bax

p53蛋白表达的影响。方法: 制备温下方含药血清

高效液相色谱法(HPLC)定性检测含药血清中人参皂苷Rb1、大黄素和乌头碱。A549/DDP细胞分为DDP干预组

含药血清＋DDP干预组

正常血清＋DDP干预组。孵育24 h后

流式细胞仪检测细胞凋亡率

激光共聚焦显微镜检测Bcl-2

Bax及p53蛋白表达。结果: HPLC结果显示含药血清中含有人参皂苷Rb1、大黄素及乌头碱。含药血清作用后

与DDP干预组比较

含药血清+DDP干预组细胞凋亡率增高至(21.82±3.27)% (P<0.05);细胞内Bcl-2蛋白表达降低至2.81±0.02 (P<0.05);Bax蛋白表达增高至2.90±0.08 (P<0.05);p53蛋白表达增高至3.58±0.09 (P<0.05)。结论: 温下方含药血清明显诱导A549/DDP细胞凋亡

显著降低Bcl-2蛋白表达

提高Bax

p53蛋白表达

可能从诱导耐药细胞凋亡角度逆转耐药。

Abstract

Objective: To investigate the effect of Wenxia recipe medicated serum on inducing apoptosis of A549/DDP cells and expression of Bcl-2

Bax and p53 protein by using serum pharmacological methods in vitro. Method: Drug-containing serum was prepared and analyzed by HPLC. Furthermore

A549/DDP cells were divided into DDP group

drug-containing serum + DDP group and normal serum +DDP group.After incubated 24h

cell apoptosis was evaluated by laser flow cytometry and the expression of Bcl-2

Bax and p53 protein in cells was investigated by laser scanning confocal microscope. Result: There were ginsenoside Rb1

emodin

aconitine in drug-containing serum. Compared to DDP group

the apoptosis rate of drug-containing serum + DDP group rose to (21.82±3.27)% (P<0.05)

the expression of Bcl-2 significantly dropped to 2.81±0.02 (P<0.05) and the expression of Bax and p53 significantly rose to 2.90±0.08 and 3.58±0.09 (P<0.05) respectively. Conclusion: Wenxia recipe medicated serum could significantly induce apoptosis of A549/DDP

decrease the expression of Bcl-2 and increase the expression of Bax and p53.This study showed that Wenxia recipe might reverse the mutidrug-resistance of A549/DDP cell by inducing apoptosis.

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