Objective: To investigate the immune regulatory effect of Wuzi Yanzong decoction on immunosuppression mice. Method: Thirty two Balb/C mice

half male and femal

were divided into four groups.Three groups of mice were administered Cy ip (50 mg ·kg-1 once a day for 5 days)to induce immunosuppression; two of these groups received Wuzi Yanzong decoction treatment (2

4 g ·kg-1 orally once a day for 35 days; 5 days after Cy ip).A vehicle treated control group was also included.The body weight and spleen weight were measured.The absolute number of total splenocytes and bone marrow cells was analyzed.The absolute number of CD3+T cells

CD4+T cells

CD8+ T cells

B cells and NK cells in spleen was detected by flow cytomety. Result :Cy-treated mice showed a significant decrease in the number of leukocytes on peripheral blood

total splenocytes

bone marrow cells and the absolute number of CD3+T cells

CD4+T cells

CD8+ T cells

B cells and NK cells in spleen.Wuzi Yanzong decoction significantly increased the number of leukocytes in peripheral blood

antagonist the loss of immune organ and body weight

restore the number of leukocytes

bone marrow nucleated cells

total splenocytes and improved the ratio and number of CD3+T cells

CD4+T cells

CD8+ T cells

B cells and NK cells in spleen. Conclusion: Wuzi Yanzong decoction markly improves the immune function in immunodepressed mice induced by Cy.