Objective: To investigate pharmacokinetics of Xiongbing Microemulsion in rabbit blood. Method: The plasma concentration of tetramethylpyrazine (TMP) was determined by HPLC after i.v.The data were processed by DAS 2.1 software to calculate the pharmacokinetic parameters. Result: The calibration curve was linear in the range of 0.062-12.4 mg ·L-1 (R2=0.998).The measured pharmacokinetics of free TMP concentration fitted open two compartment model after i.v.The main pharmacokinetic parameters for TMP were as follows:t1/2α was 15.617 min;t1/2was 49.171 min;and V was 0.509 L ·kg-1;CL was 0.014 L ·min-1 ·kg-1;AUC(0-∞)was 222 063.235 μg ·min ·L-1;MRT (0-∞)was 30.841 min. Conclusion: With good linearity

precision and recovery

the method is sensitive and simple

and suitable for pharmacokinetic study and the research of TMP.The pharmacokinetic characteristics of TMP in rabbits were understood.