野生绵茵陈和花茵陈中绿原酸含量的比较

胡彦武; 关颖丽; 姚慧敏; 连丹

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野生绵茵陈和花茵陈中绿原酸含量的比较

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- 野生绵茵陈和花茵陈中绿原酸含量的比较
- Comparison on Chlorogenic Acid Content of from Different Harvest Time
- 中国实验方剂学杂志 2011年17卷第9期页码：78-80
- 作者机构：
- 作者简介：
- 基金信息：
  
  通化师范学院自然科学科研基金项目(201076)
- DOI：
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- 纸质出版日期：2011
- 稿件说明：
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胡彦武, 关颖丽, 姚慧敏, 等. 野生绵茵陈和花茵陈中绿原酸含量的比较[J]. 中国实验方剂学杂志, 2011,17(9):78-80.

HU Yan-wu, GUAN Ying-li, YAO Hui-min, et al. Comparison on Chlorogenic Acid Content of from Different Harvest Time[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 78-80.
胡彦武, 关颖丽, 姚慧敏, 等. 野生绵茵陈和花茵陈中绿原酸含量的比较[J]. 中国实验方剂学杂志, 2011,17(9):78-80. DOI：

HU Yan-wu, GUAN Ying-li, YAO Hui-min, et al. Comparison on Chlorogenic Acid Content of from Different Harvest Time[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 78-80. DOI：

摘要

目的: 采用超声提取法提取绵茵陈和花茵陈中绿原酸并用HPLC测定其含量。方法: Agilent ZORBAX Eclispse XDB C18(4.6 mm×150 mm

5 μm)色谱柱

乙腈-0.4%磷酸溶液(15:85)为流动相

柱温25 ℃

流速0.8 mL ·min－1

检测波长327 nm。结果: 绿原酸在0.103~5.130 μg呈良好的线性关系(r=0.999 8)

平均回收率98.22%

RSD 1.72%。测得绵茵陈、花茵陈中绿原酸质量分数分别为0.81%

0.46%。结论: 野生绵茵陈和花茵陈均含有绿原酸

以绵茵陈中绿原酸含量较高。

Abstract

Objective: To extract and determine chlorogenic acid from Artemisia capillaries in different harvest times with ultrasonic extraction and HPLC. Method: The separation was accomplished an Agilent ZORBAX Eclispse XDB C18 (4.6 mm×150 mm

5 μm) with a mixture of acetonitrile-0.4% H3PO4 (15 ∶85) as the mobile phase

the column temperature was at 25 ℃; the flow rate was 0.8 mL ·min-1; the detection wave length was at 327 nm. Result: The linear range of rutin was 0.103-5.130 μg (r=0.999 8). The average recovery was 98.22% with RSD of 1.72%. The content of chlorogenic acid of A.capillaries from two harvest times was 0.81% and 0.46%. Conclusion: A.capillaries in different harvest times all contain chlorogenic acid and the content was higher in A.capillaries seedlings.

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