Objective: To study the preparation process of extraction and purification for analgesic and hemostatic bioactive portion from Lamiophlomis rotata. Method: Determination of the total iridoid glycosides and 8-epideoxyloganic acid were performed by dimethylaminobenzaldehyde method and HPLC respectively. By Using single factor and orthogonal experiments

the best extraction and purification process with macroporous resin was optimized. Result: The best extraction was established as follows: solvent of 60% ethanol

soaking 36 hours

elution volume was 10 times of materials

percolation rate of 5mL ·min-1kg-1. The purification process was as follows: the ethanolic extract should be separated by polyamide. The water elution was separated with XDA-7 macroporous resin and collecting the 30% ethanolic extract. Conclusion: XDA-7 macroporous resin has better ability of absorption and anti-absorption for iridoid glycosides and 30% ethanol was the best solvent for anti-absorption.