Objective: To establish a HPLC fingerprint for the identification of Smilax china. Method: The analysis was carried out on a Diamonsil C18(4.6 mm×250 mm

5 μｍ)with a mobile phase consisting of acetonitrile-0.1 % phosphoric acid

gradient eluent

the flow rate of 0.6 mL ·min-1. The wave length was 330 nm

data were analyzed by fingerprint similarity evalution software to compare the similarity of samples. Result: The mutual mode to HPLC fingerprints was estabilished

and the 18 mutual peaks were indicated.The similarities among the of S. china and S. glabra collected from different sources were obtained. there were apparent difference in fingerprint. Conclusion: HPLC fingerprint method is repeatable and feasible and can be suitable for identifying Musa basjoo.