YAO Lin, WANG Wei-ming, ZHANG Yan-yan, et al. Studies on Extraction and Purification Technology of Chinese Medicine Leeches[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 31-33.
YAO Lin, WANG Wei-ming, ZHANG Yan-yan, et al. Studies on Extraction and Purification Technology of Chinese Medicine Leeches[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 31-33.DOI:
Objective: Studies on extraction and purification technology of the Chinese medicine leeches
preparing process of extracting leeches to provide technical basis. Method: The extraction of leeches was optimized by orthogonal experiment and countercurrent circuit extractive technique
with titer detected by anti-thrombin activity as an index
and using of membrane separation technology to refine extract. Result: The best technology was to extract five hours with 12 times the amount of 0.9% sodium chloride solution
and extract three times. Selected membrane with 30×103 and 0.5×103 could achieve the purpose of the purification. Conclusion: The extraction and purification technology was stable