Objective: To investigate the effects of the Shenshuai Baoshen Capsule on extracellular matrix accumulation in rat renal interstitial fibroblast

and analyze the mechanisms of the kidney protection effects of the Shenshuai Baoshen Capsule. Method: Sixty Wistar rats were randomly divided into blank control group

model control group

Shenshuai Baoshen capsule low dose group and high dose group. The model was established by ig administration of adenine. Different groups were treated for 8 weeks

The pathlological changes in kidney functions after therapy were observed. The protein collagen Ⅰ

MMP-1(matrix metalloproteinase-1)

TIMP-1(tissue inhibitor of metalloproteinase-1)

PAI-1(plasminogen activator inhibitor) expression in kidney tissue were detected respectively by immunohistochemistry. Result: The pathlological changes were reduced in Shenshuai Baoshen capsule group

Cr

BUN and UA were reduced

the expression of collagen Ⅰ

TIMP-1

PAI-1 protein in kidney tissue were reduced

and the expression of MMP-1 was induced which had the significant difference compared with that in the model group(P<0.01

P<0.05). Conclusion: The Shenshuai Baoshen Capsule could depress the collagen Ⅰprotein accumulation in renal tissue

by rising MMP-1 expression

depressing TIMP-1

PAI-1 protein expression. Therefore

the renal interstitial fibroblast was relieving by Shenshuai Baoshen Capsule

and chronic renal failure progress was delayed.