Objective: To establish the HPLC fingerprint of Medulla Junci for its quality evaluation. Method :HPLC fingerprint was achieved on an Agilent HC-C18 column with methanol-water as mobile phase in gradient elution and 254 nm as detection wavelength. The time for analysis was 40 minutes. Similarity of HPLC fingerprint was performed on the Similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine. Result: Eleven samples were analyzed. The common pattern of HPLC fingerprint of Medulla Junci consisted of 16 peaks. The rate of peak area of dehydroeffusol

a bioactive compound of Medulla Junci.

was from 45.14% to 67.86% in the fingerprints of 11 samples. The HPLC fingerprints of 11 samples showed over 90% similarity to the common pattern of HPLC fingerprint. Conclusion: The fingerprints of Medulla Junci on market have high similarity

indicating a high chemical homogeneity of Medulla Junci. HPLC fingerprint can be used in quality evaluation of Medulla Junci.