ZHANG Lu, DAI Long, YANG Tian-yi, et al. Optimization of Extraction Process of Collagen from Pigskin by Enzyme Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 8-11.
ZHANG Lu, DAI Long, YANG Tian-yi, et al. Optimization of Extraction Process of Collagen from Pigskin by Enzyme Hydrolysis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 8-11.DOI:
酶解法从猪皮中提取胶原的工艺优选
摘要
目的 :筛选蛋白酶酶解猪皮制备胶原的最佳工艺; 方法: 以胶原得率为指标
采用单因素试验及正交试验对酶解条件进行优选; 结果: 胃蛋白酶用量为3%
酶解液pH 2.0
底物质量浓度1 ∶28
温度0 ℃
酶解时间48 h时胶原的得率最高; 结论: 胃蛋白酶酶解猪皮制备胶原的工艺较好。
Abstract
Objective: Screen the optimum preparation for collagen by enzyme hydrolysis. Method: Enzymatic condition was optimized by single-factor experiments and the orthogonal design with collagen yield as the index. Result: The optimum extraction condition was obtained as follows:the ratio of enzyme/substrate was 3%
pH 2.0
material-liquid ratio 1 ∶28
enzymatic hydrolyzed for 48 h at 0 ℃. Conclusion: Preparation for pigskin collagen by pepsin is better.