Objective: To investigate the effect of wogonin on the proliferation and cell cycle of the SPC-A-1 cell line. Method :The cultured SPC-A-1 cells were treated with different concentrations of wogonin. The proliferation of SPC-A-1 cells was detected by MTT method. Cell apoptosis and cell cycle were detected by AO+EB straining and flow cytometry. Result :After wogonin administration

SPC-A-1 cells proliferation inhibition ratio and apoptosis ratio increased significantly in a dose-dependent manner. AO+EB straining showed partial cells presented characteristic morphological changes of apoptosis.SPC-A-1 cells showed G0/G1 phase blockage when the concentration of wogonin was 10

20 mg ·L-1

and S phase blockage appeared when the concentration of wogonin was 30

40 mg ·L-1. Conclusion :Wogonin can inhibit the proliferation of SPC-A-1 cells and induce apoptosis

also possess the effect of G0/G1 and S phase blockage and potential antitumor effect.