Objective: The fatty acid components from the rhizome of Tupistra chinensis produced in Hanzhong and Liuba of the Qinling-Daba mountains in Shaanxi province were extracted by soxhlet extraction. Method: The experiment were identified and determined by gas chromatography-mass spectrometry(GC-MS) after methyl esterification treatment. Result: The relative percentage of each component was confirmed by peak-area normalization method. Total 29 kinds of fatty acid were identified from T. chinensis produced in Liuba on Southern Slope of Qinling mountains. The main components of fatty acids were hexadecanoic acid(42.815%)

9

12-octadecadienoic acid(11.386%)

9-octadecenoic acid(6.378%)

octadecanoic acid

8

10-dimethoxyl(6.355%)

octadecanoic acid(4.18%)

octadecanoic acid

9

10

12-trimethoxyl(3.724%)

etc. Total 31 kinds of fatty acid were identified from T.chinensis in Nanzheng on Northern slope of Bashan mountain. The main components of fatty acids were hexadecanoic acid(32.404%)

9

12-octadecadienoic acid(10.144%)

hexacosanoic acid(5.466%)

octadecanoic acid

8

10-dimethoxy(5.418%)

9-octadecenoic acid(4.675%)

octadecanoic acid(4.664%)

octadecanoic acid

9

10

12-trimethoxyl(4.638%)

octadecanoic acid

9

11

13-trimethoxyl(4.422%)

docosanoic acid(4.234%)

etc. Conclusion: It was shown that there were some impotent fatty acids in T.chinensis which have potential development and application value in food nutrition and health care.