Objective: To establish the quality standard of Huoxue Yangyin granules composed of Herb Leonuri

Radix Puerariae Lobatae. Method: The quality of each raw material was identified by TLC. The quantity of the preparation was conducted by HPLC and the method was developed with the condition as follows: Alltima C18 (4.6 mm×250 mm

5 μm)

mobile phase methanol-0.3% phosphoric acid (26 ∶74)

the temperature of column 35 ℃

flow rate 1.0 mL ·min-1

calculating the content with external standard method. Result: Herb Leonuri

Radix Puerariae Lobatae could be detected by TLC. Puerarin was linear in a range of 0.041 8~1.002 2 μg (r=0.999 9). The average recovery was 99.4%

and RSD was 1.4% (n=6). Conclusion: This method is simple

reliable

accurate

and can be used to control the quality of Huoxue Yangyin granules.