Objective: To study the effect of Polysaccharides of Banana Peel on proliferation

colony formation and expression of nuclear factor- kappa B(NF-κB)p65 in human breast cancer cells. Method: The MTT assay was used

and the human breast cancer cells (MCF-7) in logarithmic phase of growth were randomly divided into drug group and control group

with the concentration of 0.5

1.5

2.5

3.5 g ·L-1 Banana Peel polysaccharides in MCF-7 cells

Intervention durations were 24

48

72 h accordingly.The colony formation assay and the western blot technique were used to observe the effects of Polysaccharides on human breast cancer cell colony formation and expression of NF-κB(p65) in breast cancer cells. Test set of drug group and control group

drug group MCF-7 cell with the concentration of 0.5

1.5

2.5 g ·L-1 Banana Peel polysaccharides intervention 48 h. Result: Banana Peel polysaccharides inhibited cell proliferation and induced apoptosis of tumor cells within the drug concentration and drug effects duration of studies. MCF-7 cell colony formation rate

NF-κB (p65) protein expression decreased significantly with a dose-response manner Conclusion: Banana peel polysaccharides inhibited the MCF-7 cells proliferation and colony formation (P<0.05). Polysaccharide banana peel down-regulated the expression levels of NF-κB(p65) protein in MCF-7 cells. The mechanism of MCF-7 apoptosis may be related to down-regulation of the expression levels of NF-κB(p65).