中药加味四逆散对血吸虫病肝纤维化小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的影响

蔡锐; 李珊; 伍参荣; 胡海平

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中药加味四逆散对血吸虫病肝纤维化小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的影响

更新时间：2024-01-04

- 中药加味四逆散对血吸虫病肝纤维化小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的影响
- Effect of Compound Modified Sini Powder on Expression of Type Ⅰ and Type Ⅲ Collagen in Schistosoma Japonicum-infected Mice
- 中国实验方剂学杂志 2010年16卷第16期页码：178-181
- 作者机构：
- 作者简介：
- 基金信息：
  
  湖南省科技厅资助项目(04sk1007-26)
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  中图分类号：
- 纸质出版日期：2010
- 稿件说明：
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蔡锐, 李珊, 伍参荣, 等. 中药加味四逆散对血吸虫病肝纤维化小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的影响[J]. 中国实验方剂学杂志, 2010,16(16):178-181.

CAI Rui, LI Shan, WU Can-rong, et al. Effect of Compound Modified Sini Powder on Expression of Type Ⅰ and Type Ⅲ Collagen in Schistosoma Japonicum-infected Mice[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 178-181.
蔡锐, 李珊, 伍参荣, 等. 中药加味四逆散对血吸虫病肝纤维化小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的影响[J]. 中国实验方剂学杂志, 2010,16(16):178-181. DOI：

CAI Rui, LI Shan, WU Can-rong, et al. Effect of Compound Modified Sini Powder on Expression of Type Ⅰ and Type Ⅲ Collagen in Schistosoma Japonicum-infected Mice[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 178-181. DOI：

摘要

目的: 观察中药复方加味四逆散水煎剂对血吸虫病小鼠肝纤维化形成的影响。方法: NIH白鼠经皮肤人工感染日本血吸虫尾蚴造模

随机分为4组:B组(模型组);C组(吡喹酮组);D组(吡喹酮+加味四逆散组);E组(吡喹酮+秋水仙碱组)

同时设A组(正常对照组)。病理证实小鼠肝组织纤维化形成后的第21 d开始给药治疗

第45 d时各治疗组分别予以吡喹酮300 mg ·kg-1一次性灌胃进行杀虫处理

第78 d处死小鼠

取肝组织切片HE染色观察病理变化

免疫组化法检测肝组织Ⅰ型、Ⅲ型胶原。结果: 各组小鼠肝组织Ⅰ型胶原积分光密度均值比较:加味四逆散组明显低于模型组、吡喹酮组及秋水仙碱组(P<0.05)。加味四逆散组、秋水仙碱组Ⅲ型胶原的表达明显低于模型组、吡喹酮组(P<0.05)。结论: 中药加味四逆散联合吡喹酮能改善血吸虫病小鼠肝组织病理

降低小鼠肝组织Ⅰ型胶原、Ⅲ型胶原的表达。

Abstract

Objective: To observe the effect of compound modified SiNi powder(JWSNS) on liver fibrosis in Schistosoma japonicum-infected Mice. Method: The NIH mice were successfully induced to liver fibrosis by infection with S. japonicum and then randomly divided into groups B

D and E.The mice in groups C

D and E were treated with praziquantel

praziquantel plus JWSNS and praziquantel plus colchicine

respectively.In addition

a group of unfected NIH mice(group A) served as normal control.Histological specimens were stained with HE.Expression of type Ⅰ and type Ⅲ collagen were determined by Immunohistochemistry(IHC). Result: The protein levels of type Ⅰ and type Ⅲ collagen in praziquantel plus JWSNS group(group D) were significantly lower than those in model group(grop B)

praziquantel group(group C) and praziquantel plus colchicine group(group E)(P<0.05).These findings showed JWSNS can reduced the hepatic expression of type Ⅰ and type Ⅲ collagen in mice with liver fibrosis. Conclusion: JWSNS combined with praziquantel can decrease the expression of type Ⅰ and type Ⅲ collagen and implove liver fibrosis due to S. japonicum.

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