Objective: To investigate the factors of passive cutaneous anaphylaxis(PCA) in rats to select experimental conditions and improve the accuracy of forecasts for experiments. Method: Egg albumin and bovine serum albumin were selected as test antigens for the PCA. PCA experiments were carried out in different experimental conditions (with or without kasa agents

allergens means

rat strains

the optimal time for preparing antiserum of blood withdrawn

the difference in immunogenicity of egg albumin and bovine serum albumin

etc.). Result: ① Adjuvant could significantly enhance the sensitivity of immune response. in SD and Wistar tast.② Intraperitoneally and subcutaneously injection sensitization both could get good response of PCA

but intraperitoneally injection with adjuvant could induce chronic inflammation of abdominal cavity. ③ Sensitivity of immune response to antigen was similar in SD and Wistar rats. However

to use different strains of rats with PCA were better than to use single stain of experimental rats. ④ Content of IgE in serum after 16 days sensitization was higher than that in 14 days sensitization. ⑤ Egg albumin was superior to bovine serum albumin in immunogenicity of PCA test.