3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-B信号转导通路的影响

唐丹丽; 刘寨华; 隋宇; 佟林; 张华敏

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3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-B信号转导通路的影响

更新时间：2024-09-23

- 3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-B信号转导通路的影响
- Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats
- 中国实验方剂学杂志 2010年16卷第14期页码：161-164
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30701066)
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- 纸质出版日期：2010
- 稿件说明：
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唐丹丽, 刘寨华, 隋宇, 等. 3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-B信号转导通路的影响[J]. 中国实验方剂学杂志, 2010,16(14):161-164.

TANG Dan-li, LIU Zhai-hua, SUI Yu, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164.
唐丹丽, 刘寨华, 隋宇, 等. 3种不同治法方药对大鼠心肌缺血再灌注损伤核因子-B信号转导通路的影响[J]. 中国实验方剂学杂志, 2010,16(14):161-164. DOI：

TANG Dan-li, LIU Zhai-hua, SUI Yu, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164. DOI：

摘要

目的: 观察中医不同治法方药对大鼠心肌缺血再灌注损伤(I/R)核因子-кB(NF-кB)信号转导通路的影响。方法: 将60只SD大鼠随机分为5组:假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制I/R模型

应用Western blotting 及RT-PCR法检测各组大鼠心肌核因子IкB诱导激酶(NIK)、IкB激酶β(IKKβ)、核转录因子抑制蛋白(IкBα)及NF-кBp65 mRNA的表达。结果: 与模型组比较

各治疗组均能降低心肌NIK

IKKβ

NF-кBp65 mRNA水平并上调IкBα蛋白表达(P<0.01)。结论: 祛痰宽胸法、活血化瘀法及痰瘀同治法均对I/R大鼠有保护作用

其主要作用机制可能是通过抑制NIK

IKKβ蛋白表达

减少IкBα蛋白的磷酸化降解而增加其蛋白水平

从而抑制NF-кB的过度活化

发挥其对缺血再灌注损伤心肌的保护作用。

Abstract

Objective: To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion(I/R) injury in rats. Method: Sixty SD rats were divided into 5 groups randomly

sham-operated group: model group

removing phlegm and enlarging chest group

activating blood and dissolving stasis group

treating both phlegm and blood stasis group. The model of I/R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK

IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method. And the levels of NF-кBp65 mRNA were determined with RT-PCR. Result: Compared with model group

the expression of NIK

IKKβ and NF-кBp65 mRNA were lower

and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments(P<0.01). Conclusion: The removing phlegm and enlarging chest method

activating blood and dissolving stasis method

treating both phlegm and blood stasis method can protect myocardium from I/R. The mechanism of action was related to inhibiting the protein expression of NIK

IKKβ and enhancing the expression of IкBα

restraining the activation of NF-кB.

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