REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77.
REN Jie, LIU Guang-bin, ZOU Gan-peng, et al. Determination of Matrine in Shuyin Lotion by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 76-77.DOI:
Objective: To establish a method for determination of matrine in Shuyin Lotion. Method: The matrine content was determined by HPLC on inertsil ODS-SP column (4.6 mm×150 mm
5 μm) with a mobile phase of acetonitrile-water-triethylamine (3 ∶97 ∶0.2
adjusted to pH 3.5 with phosphoric acid)
the flow rate was 1.0 mL ·min-1
and the detection wavelength was set at 220 nm. Result : Matrine was linear in range of 3.9-117.0 μg (r=0.999 9). The average recovery was 101.69% with RSD of 1.84% (n=9). Conclusion : The method is simple
quick and accurate. It can be used for the quality control of Shuyin lotion.