RP-HPLC测定木香顺气丸中橙皮苷的含量

万慧杰; 王建国; 朱贺年

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RP-HPLC测定木香顺气丸中橙皮苷的含量

更新时间：2024-01-04

- RP-HPLC测定木香顺气丸中橙皮苷的含量
- HPLC Determination of Hesperidin in Muxiang Shunqi Pills
- 中国实验方剂学杂志 2010年16卷第16期页码：67-69
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- 纸质出版日期：2010
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万慧杰, 王建国, 朱贺年. RP-HPLC测定木香顺气丸中橙皮苷的含量[J]. 中国实验方剂学杂志, 2010,16(16):67-69.

WAN Hui-jie, WANG Jian-guo, ZHU He-nian. HPLC Determination of Hesperidin in Muxiang Shunqi Pills[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 67-69.
万慧杰, 王建国, 朱贺年. RP-HPLC测定木香顺气丸中橙皮苷的含量[J]. 中国实验方剂学杂志, 2010,16(16):67-69. DOI：

WAN Hui-jie, WANG Jian-guo, ZHU He-nian. HPLC Determination of Hesperidin in Muxiang Shunqi Pills[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 67-69. DOI：

摘要

目的 :建立反相高效液相色谱法测定木香顺气丸中橙皮苷含量的方法。方法: 色谱柱为Diamonsil(钻石)C18柱(4.6 mm×250 mm

5 μ);流动相乙腈-水(20 ∶80);流速为1.0 mL ·min-1;检测波长为286 nm;进样量10 μL

柱温30 ℃。结果: 线性关系为Y=6×107X+1×106

Abstract

Objective: To establish a RP-HPLC method for determination the content of hesperidin in Muxiang Shunqi Pills. Method: The analytical column was Diamonsil (diamonds) C18 column (4.6 mm×250 mm

5 μ);mobile phase was a mixture of acetonitrile-water (20 ∶80);flow rate was 1.0 mL ·min-1;the detection wavelength was set at 286 nm;injection volume 10 μL

column temperature was 30 ℃. Result: Withing the range of 0.02~6.40 μg

hesperidin showed a good linear relationship between the injection amount and peak areas(Y=6×107X+1×106

r=0.999 5)

average recovery rate was 99.15%

RSD was 1.32% respectively. Conclusion: The method is sensitive

repeatable

specific with the practicality.

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