HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸

韦建华; 李耀华; 梁臣艳; 曾万河; 黄杰芳; 甄汉深

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HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸

更新时间：2024-01-04

- HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸
- Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC
- 中国实验方剂学杂志 2011年17卷第3期页码：93-95
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- 纸质出版日期：2011
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韦建华, 李耀华, 梁臣艳, 等. HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸[J]. 中国实验方剂学杂志, 2011,17(3):93-95.

WEI Jian-hua, LI Yao-hua, LIANG Chen-yan, et al. Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 93-95.
韦建华, 李耀华, 梁臣艳, 等. HPLC测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸[J]. 中国实验方剂学杂志, 2011,17(3):93-95. DOI：

WEI Jian-hua, LI Yao-hua, LIANG Chen-yan, et al. Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 93-95. DOI：

摘要

目的 :建立桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的高效液相测定方法。方法 :采用Hypersil ODS2 C18(4.6 mm×250 mm

5 μm)色谱柱

以乙腈-0.1%磷酸为流动相

梯度洗脱

流速1.0 mL ·min-1

柱温为20 ℃

检测波长258

327 nm。结果 :原儿茶酸、绿原酸和咖啡酸分别在0.032 2~0.161 0μg

0.125 6~1.256μg和0.035 1~0.351 μg呈良好线性关系;平均回收率分别为102.1%、101.1%和101.5%。结论 :该法操作简便

分离效果理想

可用于测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的测定。

Abstract

Objective: To establish the methods for determining protocatechuic acid

chlorgenic acid and caffeic acid in Sangye dispensing granules by HPLC. Method: The column of Hypersil ODS2 C18 column (4.6 mm× 250 mm

5 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The flow rate was 1.0 mL ·min-1. The column temperature was at 20 ℃

the detection wavelength was at 258 nm

328 nm. Result: The average recovery of protocatechuic acid

chlorgenic acid and caffeic acid was 102.1%

101.1% and 101.5% respectively. Protocatechuic acid

chlorgenic acid and caffeic acid were linear in the range of 0.032 2-0.161 0 μg

0.125 6-1.256 μg and 0.035 1-0.351 μg respectively. Conclusion: The method is simple

accurate and can used to determine the content of the protocatechuic acid in mulberry formula granules.

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