HPLC测定消核灵胶囊中延胡索乙素的含量

周军; 尚强

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HPLC测定消核灵胶囊中延胡索乙素的含量

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- HPLC测定消核灵胶囊中延胡索乙素的含量
- Determination of Tetrahydropalmatine in Xiaoheling Capsule by HPLC
- 中国实验方剂学杂志 2010年16卷第18期页码：63-65
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- 纸质出版日期：2010
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周军, 尚强. HPLC测定消核灵胶囊中延胡索乙素的含量[J]. 中国实验方剂学杂志, 2010,16(18):63-65.

Zhou Jun, SHANG Qiang. Determination of Tetrahydropalmatine in Xiaoheling Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 63-65.
周军, 尚强. HPLC测定消核灵胶囊中延胡索乙素的含量[J]. 中国实验方剂学杂志, 2010,16(18):63-65. DOI：

Zhou Jun, SHANG Qiang. Determination of Tetrahydropalmatine in Xiaoheling Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 63-65. DOI：

摘要

目的 :建立以高效液相色谱法测定消核灵胶囊延胡索乙素的含量的方法。方法 :采用C18色谱柱(4.6 mm×150 mm

5 μm) ;流动相为乙腈-2%冰醋酸溶液(用三乙胺调pH 5.0) (25 ∶75);检测波长280 nm

流速1 mL ·min-1

柱温为30 ℃。结果 : 延胡索乙素的检测浓度在44.68~357.44 mg ·L-1与峰面积积分值呈良好线性关系( r=0.999 9

n=5) ;平均回收率为101.10%

RSD 2.42%(n=5)。结论 :本法简便、快速、准确

可用于消核灵胶囊的质量控制。

Abstract

Objective: To establish an HPLC method for the determination of tetrahydropalmatine in Xiaoheling capsule. Method: The HPLC separation was performed on C18 column (4.6 mm×150 mm

5 μm) with mobile phase consisted of acetonitrile -2% acetic acid solution (ajusted pH to 5.0 using triethylamine) (25 ∶75); The detective wavelength was set at 280 nm with a flow rate of 1 mL ·min-1and the column temperature was set at 30 ℃. Result : Corydalis was linear in the range of 44.68-357.44 mg ·L-1 with coefficient 0.999 9. Average recovery was 101.10% with RSD 2.42% (n=5). Conclusion : The method was simple

rapid and accurate

and applicable for the quality control of Xiaoheling capsule.

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