HPLC同时测定乳癖消颗粒中7种成分的含量

曹旦华; 张剑; 朱丹妮

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HPLC同时测定乳癖消颗粒中7种成分的含量

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- HPLC同时测定乳癖消颗粒中7种成分的含量
- Simultaneous Determination of Seven Components in Rupixiao Granule by HPLC
- 中国实验方剂学杂志 2011年17卷第6期页码：74-76
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- 纸质出版日期：2011
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曹旦华, 张剑, 朱丹妮. HPLC同时测定乳癖消颗粒中7种成分的含量[J]. 中国实验方剂学杂志, 2011,17(6):74-76.

CAO Dan-hua, ZHANG Jian, ZHU Dan-ni. Simultaneous Determination of Seven Components in Rupixiao Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 74-76.
曹旦华, 张剑, 朱丹妮. HPLC同时测定乳癖消颗粒中7种成分的含量[J]. 中国实验方剂学杂志, 2011,17(6):74-76. DOI：

CAO Dan-hua, ZHANG Jian, ZHU Dan-ni. Simultaneous Determination of Seven Components in Rupixiao Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 74-76. DOI：

摘要

目的: 建立HPLC同时测定乳癖消颗粒中芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯7种成分的含量。方法: 采用AlltimaC18色谱柱(4.6 mm× 250 mm

5 μm);乙腈-水为流动相

梯度洗脱

检测波长203 nm

柱温30 ℃

流速1.0 mL ·min-1。结果: 芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、哈巴俄苷、人参皂苷Rb1、去氢木香内酯线性范围分别为3.906 25~125 μg (r=0.999)

9.375~300 μg (r=0.999)

37.5~1 200 μg (r=1.000)

2.187 5~70 μg (r=0.999)

3.75~120 μg (r=0.999)

25~800 μg (r=0.999)

0.625~20 μg (r=1.000);平均加样回收率分别为97.95% (RSD 0.71%)

99.05% (RSD 1.11%)

100.70% (RSD 2.46%)

102.74% (RSD 0.35%)

103.06% (RSD 0.55%)

98.25% (RSD 1.68%)

100.16% (RSD 1.82%)。结论: 本方法方便、稳定、可靠

可用于乳癖消颗粒的质量控制。

Abstract

Objective: To establish a method for simultaneous determination of seven components such as paeoniflorin

ginsenoside R1

ginsenoside Rg1

forsythin

harpagoside

ginsenoside Rb

dehydrocostuslactone contained in Rupixiao granule. Method: HPLC was conducted on an Alltima C18 column (4.6 mm×250 mm

5 μm) at 30 ℃; the mobile phase was consisted of acetonitrile (A)-water (B) with gradient elution at the flow rate of 1.0 mL ·min-1 detected at 203 nm. Result: The linear range (mg ·L-1) of paeoniflorin

ginsenoside R1

ginsenoside Rg1

forsythin

harpagoside

ginsenoside Rb

dehydrocostuslactone was 3.906 25-125 (r=0.999)

9.375-300 (r=0.999)

37.5-1 200 (r=1.000)

2.1875-70 (r=0.999)

3.75-120 (r=0.999)

25-800 (r=0.999) and 0.625-20 μg(r=1.000) respectively. The average recoveries were 97.95% (RSD 0.71%)

99.05% (RSD 1.11%)

100.70% (RSD 2.46%)

102.74% (RSD 0.35%)

103.06% (RSD 0.55%)

98.25% (RSD 1.68%) and 100.16% (RSD 1.82%) respectively. Conclusion: The developed method was convenient

reliable and accurate for the quality control of Rupixiao preparation.

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