LIU Li-feng. Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 62-63.
LIU Li-feng. Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 62-63.DOI:
Objective: To establish a method of determination of bilirution in Niuhuang Zhibao Pills. Method: Bilirubin was determined by UV spectrophotometry according to Pharmacopoeia.The detection wavelength was at 533 nm. Result: The arerage levels of bilirubin in the Niuhuang Zhibao Pills was 29.8 μg one pill.Bilirubin was linear in the range of 0.009 8~0.049 8 mg ·mL-1with r=0.999 9
(n=5).The average recovery (n=6) was 98.74%(RSD 0.45%). Conclusion: The methods were proved to be simple sensitive and reproducible.It can be used for the qualitycontrol of Niuhuang Zhibao Pills.