Objective: To develop an HPLC method for determination of Buddleoside from Flos Chrysanthemi Indici. The result was compared with IR spectrums. Method: Flos Chrysanthemi Indici from various habitats were used as test sample and HPLC was used. The chromatographic separation was performed on a Diamonsil C18 (4.6 mm×250 mm

5 μm) column gradient eluted with a mobile phase of 0.5%H3PO4(A)-acetonitrile(B)

B changed from 10% to 20% in the first 10 min

in the next 20 min

when B changed from 20% to 30%

and in the last 10 min

B changed to 40%. The detection wavelength was set at 326 nm and column temperature was set at 35 ℃. The FTIR spectrums

the second-order derivative spectrogram and the two-dimensional infrared correlation spectrograms were used. Result: Buddleoside was linear within the range of 0.262- 5.24 μg (r=0.999 91). The average recovery was 98.75%

with RSD 1.02% (n=5). There were differences of samples on the second-order derivative spectrogram of Flos Chrysanthemi Indici. in 1 700- 1 400 cm-1 and the two-dimensional infrared correlation spectrograms could be classified between frequency range 1 800- 800 cm-1. Conclusion: The method was accurate

repetable and suitable to determine buddleoside from Flos Chrysanthemi Indici. The method can be used for quality control of Flos Chrysanthemi Indici. IR spectrum can also be used to identify the flavonoids from Flos Chrysanthemi Indici.