Objective: To develop quality standards of Tongbao Xiaojia mixture. Method: Thin layer chromatography (TLC) of Licorice root

Radix Scutellariae were identified according to high performance liquid chromatography (HPLC) of the main drug of chlorogenic acid determination

chromatographic column was Agilent ZORBAX Eclispse SB-Aq C18 column(4.6 mm×250 mm

5 μm) column; mobile phase acetonitrile-0.4% phosphoric acid (7∶93); flow rate 1.0 mL ·min; column temperature 30 ℃; detection wavelength 327 nm. Result: Tongbao Xiaojia Mixture Licorice root

Radix Scutellariae TLC chromatography

chromatography with reference substance corresponding location on the same color of the spots. Determination of chlorogenic acid good linear relationship

the regression equation Y=1.06×105X-1.00×104

r=0.999 9

chlorogenic acid

the average recovery was 100.47%

RSD to 1.50%. Conclusion: This method is simple

accurate

sensitive

reproducible and can be used as quality control Qingyan Liyan mixture