Objective: To compare the contents of daphnetin in the raw medical material and the different processed products of Cortex Daphnes. Method: The contents of daphnetin in 18 samples of Cortex Daphnes and its processed drugs were determined by HPLC.Chromatographic conditions included Hypersil C18 column and the mobile phase consisted of a mixture of methanol 0.5% phosphoric acid (20 ∶80).Daphnetin was detected at 327 nm. Result: Daphnetin was linear in the range of 0.102~0.510 μg with correlation coefficient 0.999 9.The average recovery was 98.09% with RSD 0.92% (n=6). The contents of daphnetin in 18 samples was big difference (0.01%~1.11%).But the change of contents was small before and after processed. Conclusion: The method of analysis was fast

accurate and practicable.The study would provide theoretical and experimental data in order to evaluate the quality of Cortex Daphnes and its processed drugs systematically.